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Image Search Results


Differential expression of circRNA by A, scatter plot, B, volcanic map, and C, cluster analysis in normal group and diabetic cardiomyopathy (DCM) group. circRNA, circular RNA.

Journal: Journal of Diabetes

Article Title: CircMAP3K5 promotes cardiomyocyte apoptosis in diabetic cardiomyopathy by regulating miR ‐22‐3p/ DAPK2 Axis

doi: 10.1111/1753-0407.13471

Figure Lengend Snippet: Differential expression of circRNA by A, scatter plot, B, volcanic map, and C, cluster analysis in normal group and diabetic cardiomyopathy (DCM) group. circRNA, circular RNA.

Article Snippet: These circRNAs were amplified and transcribed into fluorescent cRNA using a random primer method (Arraystar Super RNA Labeling Kit; Arraystar). labeled cRNAs that were hybridized to Arraystar Rat circRNA Array V2.0 (8 × 15 K) with a total of 14 145 circRNA probes on the chip.

Techniques: Quantitative Proteomics

Depletion of circRNA Mitogen‐activated protein kinase kinase kinase 5 (circMAP3K5) attenuates cardiomyocyte apoptosis in a diabetic cardiomyopathy (DCM) cell model. A, quantitative real‐time polymerase chain reaction results show expression levels of circMAP3K5 in samples of DCM ( n = 3) and control ( n = 3) cells. B, Western blots showing expression levels of Bcl‐2, Bax, and cleaved caspase‐3 proteins in the samples. C, Flow cytometry results depict the rate of apoptosis. Statistically significant difference: * p < .05 ( n = 3). circRNA, circular RNA; HG, high glucose.

Journal: Journal of Diabetes

Article Title: CircMAP3K5 promotes cardiomyocyte apoptosis in diabetic cardiomyopathy by regulating miR ‐22‐3p/ DAPK2 Axis

doi: 10.1111/1753-0407.13471

Figure Lengend Snippet: Depletion of circRNA Mitogen‐activated protein kinase kinase kinase 5 (circMAP3K5) attenuates cardiomyocyte apoptosis in a diabetic cardiomyopathy (DCM) cell model. A, quantitative real‐time polymerase chain reaction results show expression levels of circMAP3K5 in samples of DCM ( n = 3) and control ( n = 3) cells. B, Western blots showing expression levels of Bcl‐2, Bax, and cleaved caspase‐3 proteins in the samples. C, Flow cytometry results depict the rate of apoptosis. Statistically significant difference: * p < .05 ( n = 3). circRNA, circular RNA; HG, high glucose.

Article Snippet: These circRNAs were amplified and transcribed into fluorescent cRNA using a random primer method (Arraystar Super RNA Labeling Kit; Arraystar). labeled cRNAs that were hybridized to Arraystar Rat circRNA Array V2.0 (8 × 15 K) with a total of 14 145 circRNA probes on the chip.

Techniques: Real-time Polymerase Chain Reaction, Expressing, Control, Western Blot, Flow Cytometry